ABSTRACT
Cassytha filiformis, a leafless and perennial vine with small scales as a replacement of the leavesis currently being used in the treatment of various disease conditions including jaundice without standardization.Microscopical evaluation, chromatographic analysis (TLC, HPLC, LC-MS/MS), spectroscopic analyses (NMR, FTIR, MS) and hepathoprotective studies were carried out with the view to scientifically verify the potentials of this plant used in traditional medicine. The results revealed the presence of some diagnostic microscopical features such as paracytic stomata, unicellular covering trichomes with cystoliths, prismatic calcium oxalate crystals and annular xylem vessels. Quantitative physical constants include moisture contents (5.5 %), ash value (17 %), acid insoluble ash value (1 %), total tannins (27.3 %), swelling index (165 %), water, alcohol and oil extractive indices (20.6 %, 13.6 % and 1.6 % respectively). Trace metals detected in C. filiformis such as Fe (165.4279 ppm), Mn (14.4093 ppm) and Ni (2.7933 ppm) which are essential were higher than FAO/WHO (1984) permissible limit for edible plants. While others:Pb (0.0568 ppm) Zn (0.1094 ppm), Cd (0.0103 ppm) and Cu (0.0535 ppm) were found to be within the safety limit. Preliminary phytochemical screening of the plant signifies the presence of alkaloids, tannins, flavonoids, saponins and steroids Phytochemical constituents identified in ethyl acetate and methanol extracts of C. filiformis include 3,3,O-di-O-methyl ellagic acid, catechin, chalcone compounds, phydroxybenzoic acid, isorhamnetin 3-O-rutinoside or isorhamnetin 3-O- viii neohesperidoside, kaemferol 3 rutinoside and 2-{cyclohex-2-en-1- yl(hydroxyl)methyl}-3-hydroxy-4-(2-hydroxyethyl)-3-methyl- oxoprolinate while that of methanol fraction include 3,3,O-di-O-methyl etllagic acid, methy2- {cyclohex-2-ene-1-y(hydroxyl)methyl}-3-hydroxy-4-(2-hydroxyethyl)-3-methyl-5- oxoprolinate, kaemferol 3 rutinoside, rutin and cathechinas revealed by the library search on LC-MS/MS. Other compounds β-sitosterol and stigmasterol wereisolated from petroleum ether extracts.The petroleum ether extract (500 mgkg-1 ) and methanol (500 and 1000 mgkg-1 ) exhibited hepatoprotection properties on wistar albino rats. These results could serve as bases for the use of the plant in traditional medicine for the prevention of liver disorders.
ABSTRACT
This research work highlights the result of a research carried out to examine the relevance of accounting information to...
Background to the study &...
ABSTRACT
The objective of this study was to find out the effect of socio-economic status of parent on the achievement of...
Background of the study
The origins of education may be traced back to the classroom. The chalkboard was impregna...
ABSTRACT
This research work is aimed at determining the problems of married female secretaries in some parastatals in En...
ABSTRACT
This study is attempted to provide a general information and knowledge about the attitude of mothers toward...
Abstract
This study focuses on the impact of recession on economic growth in Nigeria from 1980 to 2017. The Nigerian eco...
ABSTRACT
The broad aim of this study is to examine the role of radio in rural development using Ezinihitte Loc...
Abstract
The study determines and establishes the connection between internal auditing and internal con...
ABSTRACT
This study was carried out to assess child care stress among women of child bearing age s...